半乳甘露聚糖检测试剂盒, Galactomannan Assay Kit, 货号:K-GALM
中文品名:半乳甘露聚糖检测试剂盒
品名:Galactomannan Assay Kit
规格:100 次检测
应用:半乳甘露聚糖检测试剂盒适于食品和植物产品中办乳甘露聚糖的检测和分析。
原理:
检测方法:分光光度法 @340 nm
反应时间:~90min
检测限:样品重量≥1%
应用案例:
种子,碾磨粉和食品配料
方法识别:
新方法
试剂盒组成:
Bottle 1: |
缓冲溶液(25 mL, pH 8.6) 添加叠氮钠(0.02%)作为防腐剂(缓冲液A) |
|
4°C下稳定超过 2年 |
Bottle 2: |
NAD+ |
|
-20°C稳定超过 5年 |
Bottle 3: |
β-甘露聚糖酶悬浮液(A. niger; 1.1 mL) |
|
4°C下稳定超过 4年 |
Bottle 4: |
β-半乳糖苷酶(瓜尔豆胶种子)加上β-甘露聚糖酶悬浮液,2.2 mL。 |
|
4°C下稳定超过 4年 |
Bottle 5: |
β-半乳糖脱氢酶 2.4 mL。 |
|
4°C下稳定超过 2年 |
Bottle 6: |
D-半乳糖标准溶液 (5 mL, 0.4 mg/mL 溶于0.02%叠氮钠溶液中)。 |
|
4°C下稳定超过 4年 |
Bottle 7: |
长豆角半乳糖甘露聚糖粉质控品(半乳甘露聚糖含量在小瓶标签上标明) |
|
室温下稳定超过 5年 |
优点:
试剂盒内包含半乳糖脱氢酶
价格具有竞争力
所有试剂制备后可以稳定保存超过2年
仅提供酶试剂盒
操作简单
官网提供Mega-Calc™ 软件工具用于一站式原始数据处理
包含标准溶液
适合手工、微孔板和自动分析仪分析。
参考文献:
An enzymic technique for the quantitation of galactomannan in guar Seeds. McCleary, B. V. (1981). Lebensmittel-Wissenschaft & Technologie, 14, 56-59.
Measurement of total starch in cereal products by amyloglucosidase-alpha-amylase method: collaborative study. McCleary, B. V., Gibson, T. S. & Mugford, D. C. (1997). Journal of AOAC International, 80, 571-579.
Measurement of carbohydrates in grain, feed and food. McCleary, B. V., Charnock, S. J., Rossiter, P. C., O’Shea, M. F., Power, A. M. & Lloyd, R. M. (2006). Journal of the Science of Food and Agriculture, 86(11), 1648-1661.
Metabolic and genetic perturbations accompany the modification of galactomannan in seeds of Medicago truncatula expressing mannan synthase from guar (Cyamopsis tetragonoloba L.). Naoumkina, M., Vaghchhipawala, S., Tang, Y., Ben, Y., Powell, R. J. & Dixon, R. A. (2008). Plant Biotechnology Journal, 6(6), 619-631.
Enzymatic improvement of guar‐based thickener for better‐quality silk screen printing. Baldaro, E., Gallucci, M., Formantici, C., Issi, L., Cheroni, S. & Galante, Y. M. (2012). Coloration Technology, 128(4), 315-322.
Nutrient utilisation and intestinal fermentation are differentially affected by the consumption of resistant starch varieties and conventional fibres in pigs. Rideout, T. C., Liu, Q., Wood, P. & Fan, M. Z. (2008). British Journal of Nutrition, 99(05), 984-992.
使用手册:
相关产品:
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Galactomannan (Carob; High Viscosity)
Galactomannan (Guar; High Viscosity)
Galactomannan (Guar; High Visc, Gal depleted; 21% Gal)
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endo-1,4 β-Mannanase (Bacillus sp.)