产品介绍
StemSure LIF, mouse, recombinant, solution for mouse ES cell culture,StemSure白血病抑制因子,货号:195-16053,品牌:Wako
StemSure Series for culture of ES cells
StemSure Series is a group of products whose quality has been confirmed by culturing mouse ES D3 strain cells and performing a colony forming test and alkaline phosphatase staining in addition to the quality specifications for liquid media for general uses.
Source: E. coli expressed mouse leukemia inhibitory factor
Storage Buffer: D-PBS, 1% BSA
Activity: 106 units/mL
Unit Definition: One unit is one twentieth the amount of rmLIF which induces 50% cell growth of the maximum cell growth in the cell growth-promoting assay of mouse ES cells (D3).
Sterility: 0.2 μm sterile filtered
Sterility Test: to pass test
Bacteria Endotoxins: max. 10 EU/mL
Mycoplasma Test: to pass test
Practical Test (Growth of Mouse ES cells (D3)): to pass test
Alkaline Phosphatase Staining: to pass test
Working Dilution for Differentiation Inhibitory of mouse ES cells: 1,000 units/mL
106 units of StemSure rmLIF is suitable for the preparation of 1 L of ES cells culture medium.
Murine leukemia inhibitory factor (LIF) has been found as a factor differentiating mouse leukemia cell-derived M1 cells and has been reported subsequently to inhibit differentiation of embryonic stem cells (ES cells). This product is used generally in the culture of mouse ES cells as a factor maintaining undifferentiation of mouse ES cells.